ABSTRACT
Aims: We evaluated effects of extracts of both Moringa oleifera (MOR) leaves and ginger (GIN) root on dextran sodium sulphate (DSS) induced colitis mice. Study Design: experimental study Place and Duration of Study: Clinical Pharmacology dep., Mansoura Faculty of Medicine. To weeks study. Methodology: Forty BALB/c mice were used throughout this study. Mice were divided into 5 groups (n=8). Group (1) received plain filtered water. Group (2) received DSS. Group (3) received DSS and MOR. Group (4) received DSS and GIN. Group (5) received DSS plus MOR & GIN. All mice were sacrificed after 14 days of colitis induction and colon was removed. Length of the colon was detected and examined microscopically and immunohistochemistery for detection of NFKβ. Biochemical assessment of TNFα was done in serum, while, MDA and GSH were done in colonic tissue homogenate. Results: Both MOR and/ or GIN showed significant reduction in DAI, microscopic lesion score, NFKβ expression as well as significant improvement in TNFα, MDA, and GSH levels as compared to group (2). Conclusion: The ethanolic extract of leaves of MOR and/ or extract of GIN root showed significant improvement of experimentally induced colitis, which may be attributed to its anti-inflammatory and antioxidant properties. Combination therapy of GIN and MOR showed non additive benefit than GIN alone. These natural plants could be used as additive to drug therapy of IBD.
ABSTRACT
Inflammatory bowel disease (IBD) (ulcerative colitis and Crohn's disease) is a chronic intestinal inflammatory disease characterized by tissue edema, increased gut epithelial permeability, and extensive infiltration of the gut by leukocytes. Statins, in addition to their cholesterol-lowering activity, have pleiotropic effects, including immune-modulatory and anti-inflammatory effects. Simvastatin is a commonly prescribed statins with antioxidant and anti-inflammatory properties Thus; the aim of this study is to compare effect of simvastatin (5 mg/kg) and simvastatin (50 mg/kg) as an early treatment of experimentally induced ulcerative colitis in mice. For the first time in the current study, Simvastatin was administered after the appearance of signs and symptoms of the disease as an early treatment model. Twenty four mice were divided into four groups; control group, non treated DSS-induced colitis group, simvastatin (5 mg/kg/d) -treated DSS-induced colitis group, simvastatin (50 mg/kg/d) -treated DSS-induced colitis group. simvastatin at dose of (5 mg/kg/d) reduced MDA and TNF-α .While simvastatin at dose of (50 mg/kg/d) showed a significant increase in colon length of mice,a significant decrease in NO and MDA levels and a significant increase in r GSH level. Simvastatin (5 mg/kg/d) and (50 mg/kg/d) reduced the percentage of DAI by 25% and 41% respectively. The sums of histopathological scores were improved after simvastatin treatment. It can be concluded that effects of simvastatin treatment was mostly dose dependant. Unfortunately the high dose has no clinical application in human due to toxicity. So it is advised to use simvastatin with a dose of 5mg/kg as an early treatment of dss induced colitis model.